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Expression patterns of the lysophospholipid receptor genes during mouse early development
Author(s) -
Ohuchi Hideyo,
Hamada Aska,
Matsuda Hironao,
Takagi Akira,
Tanaka Masayuki,
Aoki Junken,
Arai Hiroyuki,
Noji Sumihare
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21736
Subject(s) - biology , microbiology and biotechnology , lysophosphatidic acid , g protein coupled receptor , receptor , in situ hybridization , primordium , organogenesis , tbx1 , gene , signal transduction , gene expression , genetics , promoter
Lysophospholipids (LPs) such as lysophosphatidic acid (LPA) and sphingosine‐1‐phosphate (S1P) are known to mediate various biological responses, including cell proliferation, migration, and differentiation. To better understand the role of these lipids in mammalian early development, we applied whole‐mount in situ hybridization techniques to E8.5 to E12.5 mouse embryos. We determined the expression patterns of the following LP receptor genes, which belong to the G protein–coupled receptor (GPCR) family: EDG1 to EDG8 ( S1P 1 to S1P 5 and LPA 1 to LPA 3 ), LPA 4 (GPR23 /P2Y9), and LPA 5 (GPR92). We found that the S1P/LPA receptor genes exhibit overlapping expression patterns in a variety of organ primordia, including the developing brain and cardiovascular system, presomitic mesoderm and somites, branchial arches, and limb buds. These results suggest that multiple receptor systems for LPA/S1P lysophospholipids may be functioning during organogenesis. Developmental Dynamics 237:3280–3294, 2008. © 2008 Wiley‐Liss, Inc.