Premium
Renal glomerulogenesis in medaka fish, Oryzias latipes
Author(s) -
Fedorova Svetlana,
Miyamoto Rieko,
Harada Tomohiro,
Isogai Sumio,
Hashimoto Hisashi,
Ozato Kenjiro,
Wakamatsu Yuko
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21687
Subject(s) - biology , mesonephros , oryzias , intermediate mesoderm , mesonephric duct , somitogenesis , pronephros , nephron , kidney , embryo , kidney development , hatching , in situ hybridization , duct (anatomy) , mesoderm , anatomy , glomerulus , andrology , zebrafish , microbiology and biotechnology , endocrinology , fish <actinopterygii> , embryogenesis , gene expression , embryonic stem cell , genetics , gastrulation , gene , ecology , medicine , fishery , somite
We provide an overview of glomerulogenesis in medaka from the embryo to the adult by means of in situ hybridization with the wt1 gene as a marker as well as histology and three‐dimensional images. The pronephric glomus starts to develop in the intermediate mesoderm during early somitogenesis, is completed before hatching, and persists throughout the lifetime of the fish. Within 5 days after hatching, mesonephric glomerulus formation begins in the caudomedial end of the pronephric sinus and duct area. The number of glomeruli reaches approximately 200–300 in each kidney within 2 months after hatching. wt1 expression during nephron maturation served as a marker for the formation of the mesenchymal condensate and the nephrogenic body. Existence of mesenchymal condensates and persistence of wt1 expression in the adult kidney suggest that the mesonephros retains precursor cells that may be capable of contributing to neoglomerulogenesis during adulthood. Developmental Dynamics 237:2342–2352, 2008. © 2008 Wiley‐Liss, Inc.