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Dynamic expression patterns of G protein‐regulated inducer of neurite outgrowth 1 (GRIN1) and its colocalization with Gαo implicate significant roles of Gαo‐GRIN1 signaling in nervous system
Author(s) -
Masuho Ikuo,
Mototani Yasumasa,
Sahara Yoshinori,
Asami Junko,
Nakamura Shun,
Kozasa Tohru,
Inoue Takayoshi
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21686
Subject(s) - biology , colocalization , in situ hybridization , nervous system , embryonic stem cell , microbiology and biotechnology , immunohistochemistry , central nervous system , neuroscience , gene expression , gene , genetics , immunology
GRIN1 (Gprin 1) is a signaling molecule coexpression of which with constitutively active form of Gαo can stimulate neurite extensions in Neuro2a cells, yet its in vivo roles remain elusive. Here, we examine expression profiles of GRIN1 during mouse development by in situ hybridization (ISH) and immunohistochemistry. ISH analysis revealed that GRIN1 expression was limited to the nervous system at all developmental stages tested: in the central nervous system, GRIN1 expression occurred within the entire embryonic mantle zones, while it became restricted to sets of nuclei at postnatal to adult stages. Immunohistochemistry using a GRIN1‐specific antibody demonstrated that GRIN1 colocalized with Gαo at neuronal dendrites and axons, but it was not detected in glial cells. These results suggest that Gαo‐GRIN1 pathway could mediate significant roles in neuronal migration and differentiation at embryonic stages and exert functions in wiring and/or maintenance of specific neural circuitries at postnatal to adult stages. Developmental Dynamics 237:2415–2429, 2008. © 2008 Wiley‐Liss, Inc.