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Embryonic chick corneal epithelium: A model system for exploring cell–matrix interactions
Author(s) -
Svoboda Kathy K.H.,
Fischman Donald A.,
Gordon Marion K.
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21637
Subject(s) - microbiology and biotechnology , biology , extracellular matrix , embryonic stem cell , corneal epithelium , epithelium , morphogenesis , epithelial polarity , cornea , anatomy , neuroscience , genetics , gene
In her initial research, Elizabeth D. Hay studied amphibian limb regeneration, but later switched her focus, and for the remainder of her career addressed the role of the extracellular matrix (ECM) in regulating embryonic morphogenesis. Much of that work used the embryonic chick corneal epithelial model. This review highlights many of the discoveries that she made using this model. Hay was the first to show that embryonic corneal epithelial cells produce fibrillar collagen. Her lab was among the first to demonstrate that corneal epithelial cells respond to a collagenous substrate by increasing ECM production, and that purified ECM molecules, added to cultures of epithelial sheets, induce a reorganization of the actin cytoskeleton. These data led to the first theories of cell–matrix interactions, illustrated in a ‘hands across the membrane’ sketch drawn by Hay. Recent work with the epithelial sheet model system has elucidated many of the signal transduction pathways required for actin reorganization in response to the ECM. In all, this body of work has amply supported Hay's belief that the embryonic corneal epithelium is a powerful model system for exploring the role of the ECM in regulating the cytoskeleton, in directing cell migration, and in profoundly influencing cell growth and differentiation during development. Developmental Dynamics 237:2667–2675, 2008. © 2008 Wiley‐Liss, Inc.