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Functional characterization of lmo2 ‐ Cre transgenic zebrafish
Author(s) -
Wang Lei,
Zhang Yong,
Zhou Ting,
Fu YanFang,
Du TingTing,
Jin Yi,
Chen Yi,
Ren ChunGuang,
Peng XiaoLan,
Deng Min,
Liu Ting Xi
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21630
Subject(s) - biology , transgene , cre recombinase , zebrafish , genetically modified mouse , microbiology and biotechnology , haematopoiesis , fate mapping , progenitor cell , cre lox recombination , gene , genetics , stem cell
Abstract Cre/loxP system is a powerful tool to manipulate the genome. Transgenic animals expressing Cre recombinase in specific tissues or cells have been widely used for conditional gene targeting, lineage tracing, and other genetic analyses. In zebrafish, the transgenic line with stable expression of Cre in specific tissues and cell subtypes has not been generated and its functional activity remains to be defined. Here we report the establishment of a stable transgenic fish Tg ( zlmo2:Cre ), which specifically expresses Cre in the primitive hematopoietic progenitors and vascular endothelial cells, under the control of lmo2 promoter. Our result shows that the Cre expression pattern recapitulates the endogenous lmo2 expression pattern during embryogenesis. Crossing of the Tg ( zlmo2:Cre ) line with another established transgenic reporter line Tg ( zlmo2:loxP ‐ DsRed ‐ loxP ‐ EGFP ), induces a robust recombination activity in hematopoietic progenitors and vascular endothelial cells. Thus, the Tg ( zlmo2:Cre ) transgenic line provides an invaluable tool to dissect genetic pathways in hematopoietic development and diseases. Developmental Dynamics 237:2139–2146, 2008. © 2008 Wiley‐Liss, Inc.