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Expression of the diaphanous‐related formin proteins mDia1 and mDia2 in the rat testis
Author(s) -
Mironova Ekaterina,
Millette Clarke F.
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21622
Subject(s) - formins , biology , sertoli cell , microbiology and biotechnology , spermatogenesis , actin , germ cell , cytoskeleton , messenger rna , actin cytoskeleton , cell , genetics , endocrinology , gene
Cytoskeletal alterations in both Sertoli cells and germ cells are important during many facets of mammalian spermatogenesis. Diaphanous‐related formin proteins are known to control many aspects of actin‐based cytoskeletal rearrangements, yet nothing is known regarding the expression of formins in the testis. Accordingly, here we present the first data describing mDia1 and mDia2 mRNA and protein expression in primary Sertoli cell isolates, established tissue culture cell lines often used as models for Sertoli cell analysis, and mixed populations of adult rat male germ cells. Furthermore, we have examined intact sections of rat testis. The results suggest strongly that mDia1 and mDia2 are indeed involved in the regulation of Sertoli cell and germ structure during mammalian spermatogenesis, and provide strong indications of the future directions for mechanistic studies. Developmental Dynamics 237:2170–2176, 2008. © 2008 Wiley‐Liss, Inc.