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Maintenance of undifferentiated mouse embryonic stem cells in suspension by the serum‐ and feeder‐free defined culture condition
Author(s) -
Tsuji Yukiiko,
Yoshimura Naoko,
Aoki Hitomi,
Sharov Alexei A.,
Ko Minoru S.H.,
Motohashi Tsutomu,
Kunisada Takahiro
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21617
Subject(s) - biology , embryonic stem cell , microbiology and biotechnology , suspension culture , induced pluripotent stem cell , cell culture , kosr , regenerative medicine , stem cell , limiting , cell therapy , chemically defined medium , immunology , gene , genetics , in vitro , mechanical engineering , engineering
The proven pluripotency of ES cells is expected to allow their therapeutic use for regenerative medicine. We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium contains polyvinyl alcohol (PVA), free of either animal‐derived or synthetic serum, and contains very low amounts of peptidic or proteinaceous materials, which are favorable for therapeutic use. ES cells showed sustained proliferation in the suspension culture, and their undifferentiated state and pluripotency were experimentally verified. DNA microarray analyses showed a close relationship between the elevated expression of genes related to cell adhesions. We suggest that this suspension culture condition provides a better alternative to the conventional attached cell culture condition, especially for possible therapeutic use, by limiting the exposure of ES cells to feeder cells and animal products. Developmental Dynamics 237:2129–2138, 2008. © 2008 Wiley‐Liss, Inc.