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Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines
Author(s) -
Nielsen Sonja K.,
Møllgård Kjeld,
Clement Christian A.,
Veland Iben R.,
Awan Aashir,
Yoder Bradley K.,
Novak Ivana,
Christensen Søren Tvorup
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21610
Subject(s) - cilium , biology , hedgehog , hedgehog signaling pathway , gli2 , smoothened , microbiology and biotechnology , pancreas , pancreatic cancer , pancreatic duct , signal transduction , sonic hedgehog , endocrinology , medicine , cancer research , cancer , genetics
Hedgehog (Hh) signaling controls pancreatic development and homeostasis; aberrant Hh signaling is associated with several pancreatic diseases. Here we investigated the link between Hh signaling and primary cilia in the human developing pancreatic ducts and in cultures of human pancreatic duct adenocarcinoma cell lines, PANC‐1 and CFPAC‐1. We show that the onset of Hh signaling from human embryogenesis to fetal development is associated with accumulation of Hh signaling components Smo and Gli2 in duct primary cilia and a reduction of Gli3 in the duct epithelium. Smo, Ptc, and Gli2 localized to primary cilia of PANC‐1 and CFPAC‐1 cells, which may maintain high levels of nonstimulated Hh pathway activity. These findings indicate that primary cilia are involved in pancreatic development and postnatal tissue homeostasis. Developmental Dynamics 237:2039–2052, 2008. © 2008 Wiley‐Liss, Inc.