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WNT signaling affects gene expression in the ventral diencephalon and pituitary gland growth
Author(s) -
Potok Mary Anne,
Cha Kelly B.,
Hunt Andrea,
Brinkmeier Michelle L.,
Leitges Michael,
Kispert Andreas,
Camper Sally A.
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21603
Subject(s) - biology , diencephalon , pituitary gland , ectopic expression , endocrinology , lobe , anatomy , mutant , immunohistochemistry , medicine , microbiology and biotechnology , gene , hypothalamus , hormone , immunology , biochemistry
WNT5A deficiency causes pituitary dysmorphology and ectopic melanotrope differentiation. Immunohistochemistry for prohormone convertase 2 (PC2) marks melanotrope cells, which are normally confined to the intermediate lobe of the pituitary gland. Wnt5a mutant mice contain ectopic melanotropes in a region of dysmorphology adjacent to the intermediate lobe. We show a coronal section from an e18.5 Wnt5a mutant pituitary gland stained with a PC2 specific antibody, developed with the chromagen diaminobenzidine, and counterstained with hematoxylin. The image is artistically arranged as a mirror image duplication with the dorsal aspect of the pituitary facing the outer edges and the ventral aspect towards the center plane. See Potok et al., Developmental Dynamics 237:1006–1020, 2008. © 2008 Wiley‐Liss, Inc.