Premium
Identification of genes involved in the ciliary trafficking of C. elegans PKD‐2
Author(s) -
Bae YoungKyung,
LymanGingerich Jamie,
Barr Maureen M.,
Knobel Karla M.
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21531
Subject(s) - cilium , intraflagellar transport , biology , microbiology and biotechnology , mutant , genetic screen , dynein , gene , mutation , genetics , microtubule
Ciliary membrane proteins are important extracellular sensors, and defects in their localization may have profound developmental and physiological consequences. To determine how sensory receptors localize to cilia, we performed a forward genetic screen and identified 11 mutants with defects in the ciliary localization ( cil ) of C. elegans PKD‐2, a transient receptor potential polycystin (TRPP) channel. Class A cil mutants exhibit defects in PKD‐2::GFP somatodendritic localization while Class B cil mutants abnormally accumulate PKD‐2::GFP in cilia. Further characterization reveals that some genes mutated in cil mutants act in a tissue‐specific manner while others are likely to play more general roles in such processes as intraflagellar transport (IFT). To this end, we identified a Class B mutation that disrupts the function of the cytoplasmic dynein light intermediate chain gene xbx ‐ 1 . Identification of the remaining mutations will reveal novel molecular pathways required for ciliary receptor localization and provide further insight into mechanisms of ciliary signaling. Developmental Dynamics 237:2021–2029, 2008. © 2008 Wiley‐Liss, Inc.