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BetaA, the major beta globin in definitive red blood cells, is present from the onset of primitive erythropoiesis in chicken
Author(s) -
Alev Cantas,
McIntyre Brendan A.S.,
Nagai Hiroki,
Shin Masahiro,
Shinmyozu Kaori,
Jakt Lars M.,
Sheng Guojun
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21510
Subject(s) - erythropoiesis , biology , globin , locus (genetics) , gene , in situ hybridization , intron , embryonic stem cell , microbiology and biotechnology , genetics , gene expression , medicine , anemia
Abstract Reflecting physiological changes in oxygen acquisition and regulatory changes in globin transcription, the makeup of globin chains in erythrocytes varies in development and disease. The relationship between the globin chain composition and erythropoietic lineages/niches is not well‐understood. Using a combination of proteomic‐, genomic‐, and intron‐based in situ hybridization analyses, we show that the transcripts and protein product of the major adult beta globin, betaA, are present as early as the major embryonic beta globins during chicken primitive erythropoiesis. A rapid rise in betaA percentage is seen from embryonic day (E) 5, reaching adult profile by E7. Our data suggest that betaA locus is active from the onset of primitive erythropoiesis and that beta globin switching during development may reflect a change in relative transcript abundance rather than a strict on/off switch in gene activation. Developmental Dynamics 237:1193–1197, 2008. © 2008 Wiley‐Liss, Inc.