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Proliferation and differentiation of intestinal stem cells during metamorphosis of the red flour beetle, Tribolium castaneum
Author(s) -
Parthasarathy R.,
Palli Subba R.
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21475
Subject(s) - biology , midgut , 20 hydroxyecdysone , microbiology and biotechnology , metamorphosis , stem cell , red flour beetle , cellular differentiation , ecdysone receptor , medicine , endocrinology , insect , larva , biochemistry , hormone , botany , transcription factor , nuclear receptor , gene
The insect midgut epithelium is remodeled during larval‐pupal metamorphosis when larval polyploid cells (LPCs) are replaced by the daughters of intestinal stem cells (ISCs). We characterized the proliferation of ISCs during midgut remodeling in the red flour beetle, Tribolium castaneum . Midgut remodeling is initiated at 96 hr after ecdysis into the final instar larval stage. Immunocytochemistry with bromodeoxyuridine and phospho‐histone H3 antibodies showed that the ISCs are the progenitors of the pupal/adult midgut epithelium and they undergo proliferation and differentiation to form new midgut epithelium. In vitro midgut culture experiments revealed that 20‐hydroxyecdysone (20E) in the absence of juvenile hormone induces proliferation of ISCs. RNA interference (RNAi) mediated silencing of ecdysone receptors (EcRA and EcRB) and ultraspiracle (USP) identified EcRA and USP but not EcRB as the proteins involved in 20E regulation of ISCs proliferation. These data show that the proliferation of ISCs is under both developmental and endocrine regulation. Developmental Dynamics 237:893–908, 2008. © 2008 Wiley‐Liss, Inc.