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Activin/nodal signaling modulates XPAPC expression during Xenopus gastrulation
Author(s) -
Lou Xin,
Li Shuangwei,
Wang Jinhu,
Ding Xiaoyan
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21456
Subject(s) - nodal signaling , gastrulation , biology , xenopus , nodal , microbiology and biotechnology , wnt signaling pathway , mesoderm , germ layer , acvr2b , signal transduction , embryo , genetics , embryogenesis , tgf beta signaling pathway , gene , transforming growth factor , embryonic stem cell , induced pluripotent stem cell
Gastrulation is the first obligatory morphogenesis during vertebrate development, by which the body plan is established. Nodal signaling is a key player in many developmental processes, including gastrulation. XPAPC has been found to exert its biological function through modifying the adhesion property of cells and interacting with other several important molecules in embryos. In this report, we show that nodal signaling is necessary and sufficient for XPAPC expression during Xenopus gastrulation. Furthermore, we isolated 4.8 kb upstream DNA sequence of Xenopus XPAPC, and proved that this 4.8‐kb genomic contig is sufficient to recapitulate the expression pattern of XPAPC from gastrula to tail bud stage. Transgene and ChIP assays indicate that Activin/nodal signaling participates in regulation of XPAPC expression through a Smad binding element within the XPAPC promoter. Concomitant investigation suggests that the canonical Wnt pathway‐activated XPAPC expression requires nodal signaling. Developmental Dynamics 237:683–691, 2008. © 2008 Wiley‐Liss, Inc.