Premium
PDGF‐B signaling is important for murine cardiac development: Its role in developing atrioventricular valves, coronaries, and cardiac innervation
Author(s) -
Van den Akker Nynke M.S.,
Winkel Leah C.J.,
Nisancioglu Maya H.,
Maas Saskia,
Wisse Lambertus J.,
Armulik Annika,
Poelmann Robert E.,
LieVenema Heleen,
Betsholtz Christer,
Gittenbergerde Groot Adriana C.
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21436
Subject(s) - neural crest , biology , heart development , maldevelopment , arteriogenesis , platelet derived growth factor receptor , periostin , endocardium , embryonic stem cell , anatomy , medicine , microbiology and biotechnology , embryo , receptor , angiogenesis , growth factor , cancer research , genetics , extracellular matrix , gene
We hypothesized that PDGF‐B/PDGFR‐β‐signaling is important in the cardiac contribution of epicardium‐derived cells and cardiac neural crest, cell lineages crucial for heart development. We analyzed hearts of different embryonic stages of both Pdgf‐b−/− and Pdgfr‐β−/− mouse embryos for structural aberrations with an established causal relation to defective contribution of these cell lineages. Immunohistochemical staining for αSMA, periostin, ephrinB2, EphB4, VEGFR‐2, Dll1, and NCAM was performed on wild‐type and knockout embryos. We observed that knockout embryos showed perimembranous and muscular ventricular septal defects, maldevelopment of the atrioventricular cushions and valves, impaired coronary arteriogenesis, and hypoplasia of the myocardium and cardiac nerves. The abnormalities correspond with models in which epicardial development is impaired and with neuronal neural crest–related innervation deficits. This implies a role for PDGF‐B/PDGFR‐β‐signaling specifically in the contribution of these cell lineages to cardiac development. Developmental Dynamics 237:494–503, 2008. © 2008 Wiley‐Liss, Inc.