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TGF‐β signaling is dynamically regulated during the alveolarization of rodent and human lungs
Author(s) -
AlejandreAlcázar Miguel A.,
MichielsCorsten Matthias,
Vicencio Alfin G.,
Reiss Irwin,
Ryu Julie,
de Krijger Ronald R.,
Haddad Gabriel G.,
Tibboel Dick,
Seeger Werner,
Eickelberg Oliver,
Morty Rory E.
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21403
Subject(s) - biology , lung , transforming growth factor , signal transduction , transforming growth factor beta , microbiology and biotechnology , morphogenesis , endoglin , receptor , smad2 protein , immunology , endocrinology , medicine , gene , genetics , stem cell , cd34
Although transforming growth factor‐beta (TGF‐β) signaling negatively regulates branching morphogenesis in early lung development, few studies to date have addressed the role of this family of growth factors during late lung development. We describe here that the expression, tissue localization, and activity of components of the TGF‐β signaling machinery are dynamically regulated during late lung development in the mouse and human. Pronounced changes in the expression and localization of the TGF‐β receptors Acvrl1, Tgfbr1, Tgfbr2, Tgfbr3, and endoglin, and the intracellular messengers Smad2, Smad3, Smad4, Smad6, and Smad7 were noted as mouse and human lungs progressed through the canalicular, saccular, and alveolar stages of development. TGF‐β signaling, assessed by phosphorylation of Smad2, was detected in the vascular and airway smooth muscle, as well as the alveolar and airway epithelium throughout late lung development. These data suggest that active TGF‐β signaling is required for normal late lung development. Developmental Dynamics 237:259–269, 2008. © 2007 Wiley‐Liss, Inc.