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Nuclear localization of the zebrafish tight junction protein nagie oko
Author(s) -
BitAvragim Nana,
Rohr Stefan,
Rudolph Franziska,
Van Der Ven Peter,
Fürst Dieter,
Eichhorst Jenny,
Wiesner Burkhard,
AbdelilahSeyfried Salim
Publication year - 2008
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21389
Subject(s) - biology , zebrafish , nuclear localization sequence , microbiology and biotechnology , nuclear protein , polarity (international relations) , nuclear export signal , cell , genetics , cell nucleus , gene , transcription factor , cytoplasm
Abstract The tight junctions‐associated MAGUK protein nagie oko is closely related to Drosophila Stardust, mouse protein associated with lin‐seven 1 (Pals1), and human MAGUK p55 subfamily member 5 (Mpp5). As a component of the evolutionarily conserved Crumbs protein complex, nagie oko is essential for the maintenance of epithelial cell polarity. Here, we show that nagie oko contains a predicted nuclear export and two conserved nuclear localization signals. We find that loss of the predicted nuclear export signal results in nuclear protein accumulation. We show that nagie oko nuclear import is redundantly controlled by the two nuclear localization signals and the evolutionarily conserved region 1 (ECR1), which links nagie oko with Par6‐aPKC. Finally, deletion forms of nagie oko that lack nuclear import and export signals complement several nagie oko mutant defects in cell polarity and epithelial integrity. This finding provides an entry point to potentially novel and unknown roles of this important cell polarity regulator. Developmental Dynamics 237:83–90, 2008. © 2007 Wiley‐Liss, Inc.