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Peroxisome biogenesis occurs in late dorsal‐anterior structures in the development of Xenopus laevis
Author(s) -
Cooper Colin A.,
Walsh Logan A.,
Damjanovski Sashko
Publication year - 2007
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21370
Subject(s) - xenopus , biology , dorsum , microbiology and biotechnology , peroxisome , biogenesis , salientia , anatomy , embryo , embryogenesis , genetics , receptor , gene
Metabolism and development are two important processes not often examined in the same context. The focus of the present study is the expression of specific peroxisomal genes, the subsequent biogenesis of peroxisomes, and their potential role in the metabolism associated with the development of Xenopus laevis embryos. The temporal and expression patterns of six peroxisomal genes (PEX5, ACO, PEX19, PMP70, PEX16, and catalase) were elucidated using RT‐PCR. Functionally related peroxisomal genes exhibited similar expression patterns with their RNA levels elevated relatively late during embryogenesis. Using immunohistochemistry PMP70 and catalase protein was localized largely to dorsal‐anterior structures. Peroxisomal function was assayed with peroxisomal targeted‐GFP, which when microinjected, revealed peroxisomes in dorsal‐anterior structures at stage 45. A requirement for peroxisomal function appears to be present only late in development as organogenesis is finishing, yolk stores are depleted, and ingestion commences. Developmental Dynamics 236:3554–3561, 2007. © 2007 Wiley‐Liss, Inc.