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Bone growth in zebrafish fins occurs via multiple pulses of cell proliferation
Author(s) -
Jain Isha,
Stroka Christine,
Yan Jianying,
Huang WeiMin,
Iovine M. Kathryn
Publication year - 2007
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21270
Subject(s) - biology , cell growth , cell division , bromodeoxyuridine , mitosis , zebrafish , exponential growth , microbiology and biotechnology , cell , anatomy , cell cycle , thymidine , biophysics , genetics , dna , physics , quantum mechanics , gene
Fin length in the zebrafish is achieved by the distal addition of bony segments of the correct length. Genetic and molecular data provided evidence that segment growth uses a single pulse of growth, followed by a period of stasis. Examination of cell proliferation during segment growth was predicted to expose a graphical model consistent with a single burst of cell division (e.g., constant, parabolic, or exponential decay) during the lengthening of the distal‐most segment. Cell proliferation was detected either by labeling animals with bromodeoxyuridine (during S‐phase) or monitoring histone3‐phosphate (mitosis). Results from both methods revealed that the number of proliferating cells fluctuates in apparent pulses as a segment grows (i.e., during the growth phase). Thus, rather than segment size being the result of a single burst of proliferation, it appears that segment growth is the result of several pulses of cell division that occur approximately every 60 microns (average segment length ∼ 250 microns). These results indicate that segment lengthening requires multiple pulses of cell proliferation. Developmental Dynamics 236:2668–2674, 2007. © 2007 Wiley‐Liss, Inc.