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Identification of Lmo1 as part of a Hox‐dependent regulatory network for hindbrain patterning
Author(s) -
Matis Christelle,
Oury Franck,
Remacle Sophie,
Lampe Xavier,
Gofflot Françoise,
Picard Jacques J.,
Rijli Filippo M.,
Rezsohazy René
Publication year - 2007
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21266
Subject(s) - hox gene , hindbrain , biology , homeobox , transcription factor , morphogenesis , microbiology and biotechnology , embryonic stem cell , genetics , regulation of gene expression , gene regulatory network , gene , embryo , gene expression
The embryonic functions of Hox proteins have been extensively investigated in several animal phyla. These transcription factors act as selectors of developmental programmes, to govern the morphogenesis of multiple structures and organs. However, despite the variety of morphogenetic processes Hox proteins are involved in, only a limited set of their target genes has been identified so far. To find additional targets, we used a strategy based upon the simultaneous overexpression of Hoxa2 and its cofactors Pbx1 and Prep in a cellular model. Among genes whose expression was upregulated, we identified LMO1 , which codes for an intertwining LIM‐only factor involved in protein–DNA oligomeric complexes. By analysing its expression in Hox knockout mice, we show that Lmo1 is differentially regulated by Hoxa2 and Hoxb2 , in specific columns of hindbrain neuronal progenitors. These results suggest that Lmo1 takes part in a Hox paralogue 2–dependent network regulating anteroposterior and dorsoventral hindbrain patterning. Developmental Dynamics 236:2675–2684, 2007. © 2007 Wiley‐Liss, Inc.