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Angiopoietin‐like protein 1 expression is related to intermuscular connective tissue and cartilage development
Author(s) -
Lai DarMing,
Tu YongKwang,
Hsieh YuHsuan,
Hsu WenMing,
Lee ChinCheng,
Cheng WeiCheng,
Hsieh FonJou,
Li Hung
Publication year - 2007
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21262
Subject(s) - biology , connective tissue , cartilage , microbiology and biotechnology , embryogenesis , perichondrium , anatomy , mesoderm , paraxial mesoderm , phenotype , pathology , embryonic stem cell , embryo , gene , genetics , medicine
Angptl1, a member of the angiopoietin‐related protein family, is known to regulate angiogenesis, but little is known of its potential role in other processes. To identify the expression pattern and possible role of angptl1 during embryogenesis, we used gene targeting to generate angptl1 ‐deficient, nLacZ knockin mice. Staining for β‐galactosidase from embryonic day 9.5 to 6 months of age revealed that angptl1 was initially expressed in the paraxial mesoderm. Expression then shifted to intermuscular connective tissue (fascial plane), joint capsules, and perichondrium (laryngo‐trachea, ribs, and long bones), but not the muscles. The vasculature, central and peripheral nervous systems, digestive, respiratory, and other major organ systems did not show any angptl1 expression. This expression pattern suggests that angptl1 is related to development of the connective tissue and cartilage. Lack of phenotype in mutant mice may be due to a functional redundancy from other related factors. Developmental Dynamics 236:2643–2652, 2007. © 2007 Wiley‐Liss, Inc.