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Subcellular distribution of importins correlates with germ cell maturation
Author(s) -
Hogarth Cathryn A.,
Jans David A.,
Loveland Kate L.
Publication year - 2007
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21238
Subject(s) - gonocyte , biology , importin , microbiology and biotechnology , nuclear localization sequence , cytoplasm , subcellular localization , sertoli cell , nuclear protein , nuclear transport , cell nucleus , endocrinology , transcription factor , genetics , spermatogenesis , gene
Importin proteins regulate access to the nucleus by recognizing and transporting distinct cargo proteins. Building on studies in Drosophila and Caenorhabditis elegans , we hypothesized that regulated expression and subcellular localization of specific importins may be linked to mammalian gonadal differentiation. We identified distinct developmental and cellular localization patterns for importins β1, α3, α4 and RanBP5 (importin β3) in fetal and postnatal murine testes using Western blotting and immunohistochemistry. Importin β1 protein is detected in selected germ and somatic cells in fetal gonads, with a striking perinuclear staining evident from embryonic day (E) 14.5 within testicular gonocytes. RanBP5 exhibits age‐ and gender‐specific subcellular localization within fetal gonads. At E12.5, RanBP5 protein is cytoplasmic in gonocytes but predominantly nuclear in oogonia, but by E14.5 RanBP5 appears nuclear in gonocytes and cytoplasmic in oogonia. In postnatal testes, importin α3 and α4 in spermatocytes, spermatids, and Sertoli cells display cytoplasmic and nuclear localization, respectively. Developmental Dynamics 236: 2311–2320, 2007.© 2007 Wiley‐Liss, Inc.