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The zebrafish vitronectin receptor: Characterization of integrin αV and β3 expression patterns in early vertebrate development
Author(s) -
Ablooglu Ararat J.,
Kang Jian,
Handin Robert I.,
Traver David,
Shattil Sanford J.
Publication year - 2007
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21229
Subject(s) - zebrafish , biology , in situ hybridization , integrin , vitronectin , microbiology and biotechnology , extracellular matrix , receptor , genetics , gene expression , gene
αVβ3 is a receptor for vitronectin and other extracellular matrix ligands, and it has been implicated in angiogenesis and osteoclast function in mammals. We have cloned full‐length cDNAs of zebrafish integrin αV ( itgαV ), and two paralogous zebrafish β3 integrins ( itgβ3.1 and itgβ3.2 ). Whole‐mount in situ hybridization analysis revealed that αV and β3.1 share overlapping expression domains in apical ectodermal ridge, ventricular myocardium, hypothalamus, posterior tuberculum, medial tectal proliferation zone, and in the odontogenic field of the bilateral pharyngeal dentitions. In contrast to β3.1 , β3.2 is transiently expressed throughout the developing embryo. In situ hybridization profiles and heterologous expression of proteins in tissue culture cells suggest that β3.1 is the major β3 paralog that associates with αV in zebrafish. Furthermore, when β3.1 expression profiles are compared to those of other potential αV partners ( β1 , β5 , and β8 ), pharyngeal dentitions appear to represent a unique expression field for αV and β3.1 . Developmental Dynamics 236:2268–2276, 2007. © 2007 Wiley‐Liss, Inc.