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A hypermorphic mouse Gli3 allele results in a polydactylous limb phenotype
Author(s) -
Wang Chengbing,
Pan Yong,
Wang Baolin
Publication year - 2007
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21082
Subject(s) - gli3 , repressor , biology , gli1 , gli2 , allele , sonic hedgehog , hedgehog signaling pathway , hedgehog , genetics , phenotype , locus (genetics) , microbiology and biotechnology , gene , gene expression
Gli3 protein processing to generate the Gli3 repressor is mediated by proteasome and inhibited by Hedgehog signaling. The Gli3 repressor concentration is graded along the anterior–posterior axis of the developing vertebrate limb due to posteriorly restricted Sonic hedgehog expression. In this study, we created a small deletion at the Gli3 locus ( Gli3 Δ 68 ), which causes a half reduction in the Gli3 repressor levels and a slightly increased activity of full‐length mutant protein in the limb. Mice homozygous for Gli3 Δ 68 develop one to two extra partial digits in the anterior of the limb, while mice carrying one copy of the Gli3 Δ 68 allele die soon after birth and display seven digits. These phenotypes are more severe than those found in mice lacking one wild‐type Gli3 allele. The expression of dHand , Hoxd12 , and Hoxd13 is anteriorly expanded in the limb, even though no up‐regulation of Gli1 and Ptc RNA expression is detected. These findings suggest that a decrease in the Gli3 repressor level in combination with an increase in Gli3 full‐length activity results in more severe digit patterning abnormalities than those caused by a loss of one wild‐type Gli3 allele. Developmental Dynamics 236:769–776, 2007. © 2007 Wiley‐Liss, Inc.