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Differential gene expression patterns in porcine nuclear transfer embryos reconstructed with fetal fibroblasts and mesenchymal stem cells
Author(s) -
Kumar B. Mohana,
Jin HaiFeng,
Kim JungGon,
Ock SunA,
Hong Yonggeun,
Balasubramanian S.,
Choe SangYong,
Rho GyuJin
Publication year - 2007
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21042
Subject(s) - biology , homeobox protein nanog , blastocyst , embryo , mesenchymal stem cell , embryonic stem cell , genomic imprinting , microbiology and biotechnology , dna methylation , andrology , somatic cell nuclear transfer , gene expression , embryogenesis , genetics , gene , induced pluripotent stem cell , medicine
The present study compared the developmental ability and gene expression pattern at 4‐cell, 8‐cell, morula, and blastocyst stages of porcine nuclear transfer (NT) embryos from fetal fibroblasts (FFs) and mesenchymal stem cells (MSCs), in vitro fertilized (IVF), and in vivo derived embryos. MSC‐NT embryos showed enhanced blastocyst formation, higher total cell number, and a low incidence of apoptosis compared to FF‐NT embryos. Alterations in the expression pattern of genes implicated in transcription and pluripotency ( Oct4, Stat3, Nanog ), DNA methylation ( Dnmt1, Dnmt3a ), histone deacetylation ( Hdac2 ), growth factor signaling, and imprinting ( Igf2, Igf2r ) and apoptosis ( Bax, Bcl2 ) regulation were observed in NT embryos. The expression of transcripts in MSC‐NT embryos more closely followed that of the in vivo derived embryos compared with FF‐NT embryos. In conclusion, MSCs with a relatively undifferentiated genome might serve as suitable donors that could be more efficiently reprogrammed to re‐activate expression of early embryonic genes in porcine NT. Developmental Dynamics 236:435–446, 2007. © 2006 Wiley‐Liss, Inc.