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Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: II. The 6‐ O ‐sulfotransferase family
Author(s) -
Cadwallader Adam B.,
Yost H. Joseph
Publication year - 2006
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20990
Subject(s) - zebrafish , biology , sulfotransferase , heparan sulfate , sulfation , gene family , gene , microbiology and biotechnology , gene expression , genetics , biochemistry , glycosaminoglycan
Heparan sulfate (HS) is an unbranched chain of repetitive disaccharides, which specifically binds ligands when attached to the cell surface or secreted extracellularly. HS chains contain sulfated domains termed the HS fine structure, which gives HS specific binding affinities for extracellular ligands. HS 6‐ O ‐sulfotransferases (6‐OST) catalyze the transfer of sulfate groups to the 6‐ O position of glucosamine residues of HS. We report here the characterization and developmental expression analysis of the 6‐OST gene family in the zebrafish. The zebrafish 6‐OST gene family consists of four conserved vertebrate orthologues, including a gene duplication specific to zebrafish. We examined the mRNA expression patterns in several tissues/organs throughout early zebrafish development, including early cleavage stages, eyes, somites, brain, internal organ primordial, and pectoral fin development. Members of the 6‐OST gene family have spatially and temporally distinct restricted expression, suggesting in vivo functional differences exist between members of this family. Developmental Dynamics 235:3432–3437, 2006. © 2006 Wiley‐Liss, Inc.