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Sp5l is a mediator of Fgf signals in anteroposterior patterning of the neuroectoderm in zebrafish embryo
Author(s) -
Sun Zhihui,
Zhao Jue,
Zhang Yu,
Meng Anming
Publication year - 2006
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20945
Subject(s) - neuroectoderm , biology , zebrafish , gene knockdown , mesoderm , embryo , microbiology and biotechnology , embryonic stem cell , brachyury , fibroblast growth factor , fgf and mesoderm formation , neural plate , transcription factor , nodal , genetics , embryogenesis , ectoderm , receptor , gene
The neuroectoderm is patterned along the anterior‐posterior axis in vertebrate embryos. Fgf signals are required to induce the posterior neuroectodermal fates, but they repress the anterior fate. Sp5l/Spr2, an Sp1‐like transcription factor family member, has been shown to be required for development of mesoderm and posterior neuroectoderm. We demonstrate here that repression of the anterior neuroectodermal markers fez and otx1 by fgf17b or fgf3 coincides with induction of sp5l in the anterior neuroectoderm, and that this repression is efficiently rescued by simultaneous sp5l knockdown. On the other hand, sp5l knockdown is able to inhibit inductive activity of ectopic Fgf signals on the expression of the posterior neuroectodermal markers gbx2, hoxb1b , and krox20 . Furthermore, effect of overexpression of a dominant negative Fgf receptor on anteroposterior patterning of the neuroectoderm is rescued by sp5l overexpression. Taken together, these data suggest that sp5l mediates the functions of Fgf signals in anteroposterior patterning of the neuroectoderm during zebrafish embryogenesis. Developmental Dynamics 235:2999–3006, 2006. © 2006 Wiley‐Liss, Inc.