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Expression and function of the columnar patterning gene msh in late embryonic brain development of Drosophila
Author(s) -
Sprecher Simon G.,
Hirth Frank
Publication year - 2006
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20936
Subject(s) - biology , homeobox , embryonic stem cell , microbiology and biotechnology , embryogenesis , neural development , phenotype , gene , cell fate determination , transcription factor , regulation of gene expression , neuroscience , genetics , anatomy , embryo
In Drosophila , specification of neural identity requires a network of conserved transcription factors, such as the columnar genes for dorsoventral patterning. Here, we analyze the expression and function of the columnar patterning gene muscle specific homeobox ( msh ) in late embryonic brain development. Expression of msh is observed in all brain neuromeres, including neurons and neuropile glia. Functional analysis demonstrates that msh is essential for proper development of the tritocerebral neuromere and brain neuropile glia. Thus, msh mutants display a severe loss of neural and glial tissue together with axonal patterning defects. This gap‐like phenotype initially correlates with defects in neural and glial cell formation and during later embryonic development is associated with increased apoptotic activity. Taken together, our results provide evidence that the columnar patterning gene msh is required for correct tritocerebral neuromere development, as well as for neuropile glia formation and axogenesis in embryonic brain development of Drosophila . Developmental Dynamics 235:2920–2929, 2006. © 2006 Wiley‐Liss, Inc.