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Microarray analysis of Tbx5‐induced genes expressed in the developing heart
Author(s) -
Plageman Timothy F.,
Yutzey Katherine E.
Publication year - 2006
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20923
Subject(s) - biology , gene , heart development , tbx1 , enhancer , microarray analysis techniques , gata4 , gene expression , microarray , genetics , candidate gene , transcription factor , microbiology and biotechnology , embryonic stem cell , promoter
Tbx5 is a member of the T‐box family of transcription factors and is associated with Holt–Oram syndrome (HOS), a congenital disorder characterized by heart and limb defects. Although implicated in several processes during development, only a few genes regulated by Tbx5 have been reported. To identify candidate genes regulated by Tbx5 during heart development, a microarray approach was used. A cardiac‐derived mouse cell line (1H) was infected with adenoviruses expressing Tbx5 or β‐galactosidase and RNA was isolated for analysis using an Affymetrix gene chip representing over 39,000 transcripts. Real‐time reverse transcriptase‐polymerase chain reaction confirmed Tbx5 induction of a subset of the genes, including nppa , photoreceptor cadherin , brain creatine kinase , hairy / enhancer ‐ of ‐ split related 2 , and gelsolin . In situ hybridization analysis indicated overlapping expression of these genes with tbx5 in the embryonic mouse heart. In addition, the effect of HOS‐associated mutations on the ability of Tbx5 to induce target gene expression was evaluated. Together, these data identify several genes induced by Tbx5 that are potentially important during cardiac development. These genes represent new candidate gene targets of Tbx5 that may be related to congenital heart malformations associated with HOS. Developmental Dynamics 235:2868–2880, 2006. © 2006 Wiley‐Liss, Inc.