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Endogenous bone morphogenetic protein antagonists regulate mammalian neural crest generation and survival
Author(s) -
Anderson Ryan M.,
Stottmann Rolf W.,
Choi Murim,
Klingensmith John
Publication year - 2006
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20891
Subject(s) - biology , neural crest , bone morphogenetic protein , endogeny , bone morphogenetic protein 2 , microbiology and biotechnology , crest , bone morphogenetic protein 7 , bone morphogenetic protein 5 , medicine , endocrinology , embryo , genetics , in vitro , gene , physics , quantum mechanics
Abstract We demonstrate here that Chordin and Noggin function as bone morphogenetic protein (BMP) antagonists in vivo to promote mammalian neural crest development. Using Chrd and Nog single and compound mutants, we find that Noggin has a major role in promoting neural crest formation, in which Chordin is partially redundant. BMP signaling is increased in dorsal tissues lacking Noggin and is further increased when Chordin is also absent. The early neural crest domain is expanded with decreased BMP antagonism in vivo. Noggin and Chordin also regulate subsequent neural crest cell emigration from the neural tube. However, reduced levels of these BMP antagonists ultimately result in perturbation of neural crest cell derived peripheral nervous system and craniofacial skeletal elements. Such defects reflect, at least in part, a function to limit apoptosis in neural crest cells. Noggin and Chordin, therefore, function together to regulate both the generation and survival of neural crest cells in mammalian development. Developmental Dynamics 235:2507–2520, 2006. © 2006 Wiley‐Liss, Inc.