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Definition and spatial annotation of the dynamic secretome during early kidney development
Author(s) -
Martinez Gemma,
Georgas Kylie,
Challen Grant A.,
Rumballe Bree,
Davis Melissa J.,
Taylor Darrin,
Teasdale Rohan D.,
Grimmond Sean M.,
Little Melissa H.
Publication year - 2006
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20740
Subject(s) - biology , organogenesis , mesenchyme , kidney development , microbiology and biotechnology , morphogenesis , extracellular matrix , gene expression profiling , microarray , gene , secretory protein , gene expression , microarray analysis techniques , computational biology , genetics , mesenchymal stem cell , embryonic stem cell
The term “secretome” has been defined as a set of secreted proteins (Grimmond et al. [2003] Genome Res 13:1350–1359). The term “secreted protein” encompasses all proteins exported from the cell including growth factors, extracellular proteinases, morphogens, and extracellular matrix molecules. Defining the genes encoding secreted proteins that change in expression during organogenesis, the dynamic secretome, is likely to point to key drivers of morphogenesis. Such secreted proteins are involved in the reciprocal interactions between the ureteric bud (UB) and the metanephric mesenchyme (MM) that occur during organogenesis of the metanephros. Some key metanephric secreted proteins have been identified, but many remain to be determined. In this study, microarray expression profiling of E10.5, E11.5, and E13.5 kidney and consensus bioinformatic analysis were used to define a dynamic secretome of early metanephric development. In situ hybridisation was used to confirm microarray results and clarify spatial expression patterns for these genes. Forty‐one secreted factors were dynamically expressed between the E10.5 and E13.5 timeframe profiled, and 25 of these factors had not previously been implicated in kidney development. A text‐based anatomical ontology was used to spatially annotate the expression pattern of these genes in cultured metanephric explants. Developmental Dynamics 235:1709–1719, 2006. © 2006 Wiley‐Liss, Inc.