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GATA‐4:FOG interactions regulate gastric epithelial development in the mouse
Author(s) -
Jacobsen Christina M.,
Mannisto Susanna,
PorterTinge Susan,
Genova Elena,
Parviainen Helka,
Heikinheimo Markku,
Adameyko Igor I.,
Tevosian Sergei G.,
Wilson David B.
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20552
Subject(s) - biology , mesenchyme , gata4 , epithelium , sonic hedgehog , transcription factor , stomach , endoderm , gata transcription factor , microbiology and biotechnology , immunohistochemistry , embryonic stem cell , embryo , signal transduction , gene , genetics , immunology , gene expression , biochemistry , promoter
Transcription factor GATA‐4 is a key participant in cytodifferentiation of the mouse hindstomach. Here we show that GATA‐4 cooperates with a Friend‐of‐GATA (FOG) cofactor to direct gene expression in this segment of gut. Immunohistochemical staining revealed that GATA‐4 and FOG‐1 are co‐expressed in hindstomach epithelial cells from embryonic days (E) 11.5 to 18.5. The other member of the mammalian FOG family, FOG‐2, was not detected in gastric epithelium. To show that GATA‐4:FOG interactions influence stomach development, we analyzed Gata4 ki/ki mice, which express a mutant GATA‐4 that cannot bind FOG cofactors. Sonic Hedgehog, an endoderm‐derived signaling molecule normally down‐regulated in the distal stomach, was over‐expressed in hindstomach epithelium of E11.5 Gata4 ki/ki mice, and there was a concomitant decrease in fibroblast growth factor‐10 in adjacent mesenchyme. We conclude that functional interaction between GATA‐4 and a member of the FOG family, presumably FOG‐1, is required for proper epithelial‐mesenchymal signaling in the developing stomach. Developmental Dynamics 234:355–362, 2005. © 2005 Wiley‐Liss, Inc.