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Short‐term lineage analysis of dorsally derived Olig3 cells in the developing spinal cord
Author(s) -
Ding Lei,
Takebayashi Hirohide,
Watanabe Keisuke,
Ohtsuki Toshiaki,
Tanaka Kenji F.,
Nabeshima Yoichi,
Chisaka Osamu,
Ikenaka Kazuhiro,
Ono Katsuhiko
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20545
Subject(s) - biology , lineage (genetic) , spinal cord , term (time) , anatomy , evolutionary biology , neuroscience , microbiology and biotechnology , computational biology , genetics , gene , physics , quantum mechanics
We examined the migration and differentiation of cells expressing Olig3, a basic helix‐loop‐helix transcriptional factor, in the developing spinal cord. Distribution of Olig3 lineage cells was demonstrated with in situ hybridization and X‐gal staining in an Olig3‐lacZ knock‐in mouse. Olig3‐positive cells first appeared in the dorsal spinal cord, except for the roof plate. Some of the dorsal Olig3 lineage cells co‐expressed Islet1/2, Math1, or Brn3a, markers for dorsal interneuron. LacZ‐positive cells were observed in the ventral‐most part of the E10.5 spinal cord, suggesting that some dorsal Olig3 lineage cells migrate into the ventral‐most part by E10.5. Ventral‐ward migration of dorsal cells and contribution to commissural interneurons were substantiated by electroporation of EGFP expression plasmid in the dorsal spinal cord of chick embryo. Dorsal midline cells were also LacZ‐positive during development. These findings suggest that dorsal Olig3 cells contribute to dorsal midline cells and commissural interneurons at intermediate and ventral levels. Developmental Dynamics 234:622–632, 2005. © 2005 Wiley‐Liss, Inc.