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Transcriptome analysis of the murine forelimb and hindlimb autopod
Author(s) -
Shou Siming,
Scott Virginia,
Reed Cheryl,
Hitzemann Robert,
Stadler H. Scott
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20514
Subject(s) - forelimb , hindlimb , biology , transcriptome , gene expression , gene , anatomy , genetics
Abstract To gain insight into the coordination of gene expression profiles during forelimb and hindlimb differentiation, a transcriptome analysis of mouse embryonic autopod tissues was performed using Affymetrix Murine Gene Chips (MOE‐430). Forty‐four transcripts with expression differences higher than 2‐fold (T test, P ≤0.05) were detected between forelimb and hindlimb tissues including 38 new transcripts such as Rdh10 , Frzb , Tbx18 , and Hip that exhibit differential limb expression. A comparison of gene expression profiles in the forelimb, hindlimb, and brain revealed 24 limb‐signature genes whose expression was significantly enriched in limb autopod versus brain tissue (fold change >2, P ≤ 0.05). Interestingly, the genes exhibiting enrichment in the developing autopod also segregated into significant fore‐ and hindlimb‐specific clusters ( P ≤ 0.05) suggesting that by E 12.5, unique gene combinations are being used during the differentiation of each autopod type. Developmental Dynamics 234:74–89, 2005. © 2005 Wiley‐Liss, Inc.