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Comprehensive expression atlas of fibroblast growth factors and their receptors generated by a novel robotic in situ hybridization platform
Author(s) -
Yaylaoglu Murat Burak,
Titmus Andrew,
Visel Axel,
AlvarezBolado Gonzalo,
Thaller Christina,
Eichele Gregor
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20441
Subject(s) - biology , in situ hybridization , fibroblast growth factor , fibroblast growth factor receptor , atlas (anatomy) , embryonic stem cell , microbiology and biotechnology , receptor , messenger rna , transcriptome , computational biology , gene expression , genetics , anatomy , gene
A recently developed robotic platform termed “Genepaint” can carry out large‐scale nonradioactive in situ hybridization (ISH) on tissue sections. We report a series of experiments that validate this novel platform. Signal‐to‐noise ratio and mRNA detection limits were comparable to traditional ISH procedures, and hybridization was transcript‐specific, even in cases in which probes could have hybridized to several transcripts of a multigene family. We established an atlas of expression patterns of fibroblast growth factors ( Fgfs ) and their receptors ( Fgfrs ) for the embryonic day 14.5 mouse embryo. This atlas provides a comprehensive overview of previously known as well as novel sites of expression for this important family of signaling molecules. The Fgf / Fgfr atlas was integrated into the transcriptome database ( www.genepaint.org ), where individual Fgf and Fgfr expression patterns can be interactively viewed at cellular resolution and where sites of expressions can be retrieved using an anatomy‐based search. Developmental Dynamics 234:371–386, 2005. © 2005 Wiley‐Liss, Inc.