Premium
Expression of all Wnt genes and their secreted antagonists during mouse blastocyst and postimplantation development
Author(s) -
Kemp Caroline,
Willems Erik,
Abdo Shaaban,
Lambiv Louis,
Leyns Luc
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20408
Subject(s) - biology , wnt signaling pathway , endoderm , gastrulation , blastocyst , primitive streak , inner cell mass , nodal signaling , dkk1 , microbiology and biotechnology , neurula , trophoblast , lrp5 , andrology , embryo , genetics , embryogenesis , embryonic stem cell , gene , placenta , pregnancy , medicine , fetus
In this extensive study, real‐time reverse transcriptase‐polymerase chain reaction was used to analyze the expression levels of all 19 Wnt genes and their 11 potential antagonists in mouse blastocysts, pregastrula, gastrula, and neurula stages. By complementing these results with in situ hybridization, we revealed new expression domains for Wnt2b and Sfrp1 , respectively, in the future primitive streak at the posterior side and in the anterior visceral endoderm before the initiation of gastrulation. Moreover, the anterior visceral endoderm expresses three secreted Wnt antagonists ( Sfrp1 , Sfrp5 , and Dkk1 ) in partially overlapping domains. We also identified expression patterns for the Wnt1 , Wnt3a , Wnt6 , Wnt7b , Wnt9a , Wnt10b , and Sfrp1 genes at the blastocyst stage. In particular, the expression of Wnt1 and Sfrp1 predominantly in the inner cell mass and of Wnt9a in the mural trophoblast and inner cell mass cells surrounding the blastocoele suggests new roles for the Wnt pathway in preimplantation development. This article is the first report on the regional expression of Wnt genes in the mouse blastocyst. Developmental Dynamics 233:1064–1075, 2005. © 2005 Wiley‐Liss, Inc.