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Boundary formation in the Drosophila wing: Functional dissection of Capricious and Tartan
Author(s) -
Milán Marco,
Pérez Lidia,
Cohen Stephen M.
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20384
Subject(s) - biology , microbiology and biotechnology , extracellular , transmembrane protein , imaginal disc , compartment (ship) , intracellular , multicellular organism , cell , gene , genetics , drosophila melanogaster , receptor , oceanography , geology
Cells in multicellular organisms often do not intermingle freely with each other. Differential cell affinities contribute to organizing cells into different tissues. Drosophila limbs and rhombomeres of the vertebrate hindbrain are subdivided into compartments. Cells in adjacent compartments do not mix. The wing primordium is subdivided into dorsal (D) and ventral (V) compartments by the activity of the LIM‐homeodomain protein Apterous in D cells. The leucine‐rich repeats transmembrane proteins Capricious and Tartan have been shown to contribute to formation of the affinity boundary between dorsal and ventral compartments. Here, we have carried out a structure–function analysis of Capricious and Tartan. We present evidence that both the extracellular and intracellular domains are required for the establishment of a DV affinity boundary. Our data suggest that the extracellular domains of Capricious and Tartan may work as ligands of an unknown D cell surface protein. Their intracellular domains may be required to transduce a signal necessary for the establishment of the DV boundary. Developmental Dynamics 233:804–810, 2005. © 2005 Wiley‐Liss, Inc.