Premium
Multipotent stem cells from adult olfactory mucosa
Author(s) -
Murrell Wayne,
Féron François,
Wetzig Andrew,
Cameron Nick,
Splatt Karisha,
Bellette Bernadette,
Bianco John,
Perry Chris,
Lee Gabriel,
MackaySim Alan
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20360
Subject(s) - olfactory mucosa , biology , neurosphere , multipotent stem cell , olfactory ensheathing glia , stem cell , neurogenesis , microbiology and biotechnology , olfactory epithelium , transplantation , adult stem cell , immunology , olfactory system , progenitor cell , neuroscience , in vitro , central nervous system , olfactory bulb , endothelial stem cell , medicine , genetics
Multipotent stem cells are thought to be responsible for the generation of new neurons in the adult brain. Neurogenesis also occurs in an accessible part of the nervous system, the olfactory mucosa. We show here that cells from human olfactory mucosa generate neurospheres that are multipotent in vitro and when transplanted into the chicken embryo. Cloned neurosphere cells show this multipotency. Multipotency was evident without prior culture in vitro: cells dissociated from adult rat olfactory mucosa generate leukocytes when transplanted into bone marrow–irradiated hosts, and cells dissociated from adult mouse olfactory epithelium generated numerous cell types when transplanted into the chicken embryo. It is unlikely that these results can be attributed to hematopoietic precursor contamination or cell fusion. These results demonstrate the existence of a multipotent stem‐like cell in the olfactory mucosa useful for autologous transplantation therapies and for cellular studies of disease. Developmental Dynamics 233:496–515, 2005. © 2005 Wiley‐Liss, Inc.