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Gene trap screening as an effective approach for identification of Wnt‐responsive genes in the mouse embryo
Author(s) -
Yamaguchi Yoshifumi,
Ogura Satoshi,
Ishida Mio,
Karasawa Mika,
Takada Shinji
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20348
Subject(s) - biology , embryo , gene , wnt signaling pathway , trap (plumbing) , identification (biology) , genetics , computational biology , botany , environmental engineering , engineering
In this study, we examined whether gene trap methodology, which would be available for systematic identification and functional analysis of genes, is effective for screening of Wnt‐responsive genes during mouse development. We screened out two individual clones among 794 gene‐trapped embryonic stem cell lines by their in vitro response to WNT‐3A proteins. One gene was mainly expressed in the ductal epithelium of several developing organs, including the kidney and the salivary glands, and the other gene was expressed in neural crest cells and the telencephalic flexure. The spatial and temporal expression of these two genes coincided well with that of several Wnt genes. Furthermore, the expression of these two genes was significantly decreased in embryos deficient for Wnts or in cultures of embryonic tissues treated with a Wnt signal inhibitor. These results indicate that the gene trap is an effective method for systematic identification of Wnt‐responsive genes during embryogenesis. Developmental Dynamics 233:484–495, 2005. © 2005 Wiley‐Liss, Inc.