Premium
Cullin‐5 plays multiple roles in cell fate specification and synapse formation during Drosophila development
Author(s) -
Ayyub Champakali,
Sen Anindya,
Gonsalves Foster,
Badrinath Kishan,
Bhandari Poonam,
Shashidhara L.S.,
Krishna Sudhir,
Rodrigues Veronica
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20322
Subject(s) - biology , microbiology and biotechnology , cell fate determination , phenotype , progenitor cell , cullin , developmental biology , cell division , lineage (genetic) , genetics , gene , cell , stem cell , transcription factor , ubiquitin , ubiquitin ligase
We describe a developmental analysis of Drosophila Cullin‐5 (Cul‐5) identified from the genome sequence on the basis of its high degree of homology to vertebrate and worm sequences. The gene is expressed in a restricted manner in ectodermal cells throughout development suggesting pleiotropic functions. We decided to examine the phenotypes of Cul‐5 aberrations in two well‐studied developmental systems: the neuromuscular junction (NMJ) and the developing sensory organ. Alteration of Cul‐5 levels in motoneurons results in an increase in bouton number at the NMJ. The cells of a sensory organ on the adult notum arise from a single progenitor cell by regulated cell division. Aberrations in Cul‐5 affect different steps in the lineage consistent with a role in cell fate determination, proliferation, and death. Such phenotypes highlight the multiple cellular processes in which Cul‐5 can participate. Developmental Dynamics 232:865–875, 2005. © 2005 Wiley‐Liss, Inc.