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Lens opacity and photoreceptor degeneration in the zebrafish lens opaque mutant
Author(s) -
Vihtelic Thomas S.,
Yamamoto Yoshiyuki,
Springer Sandra S.,
Jeffery William R.,
Hyde David R.
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20294
Subject(s) - biology , lens (geology) , mutant , microbiology and biotechnology , zebrafish , retinal , retina , lens fiber , retinal degeneration , optic cup (embryology) , immunolabeling , phenotype , eye development , immunohistochemistry , genetics , immunology , biochemistry , paleontology , neuroscience , gene
The zebrafish lens opaque ( lop ) mutant was identified in a chemical mutagenesis screen. The lop mutant, which develops normally through 4 days postfertilization (dpf), exhibits several signs of lens and retinal degeneration at 7 dpf. Histology revealed disrupted lens fibers and increased numbers of nucleated cells within the mutant lens and anterior chamber. The mutant lens also exhibited aberrant epithelial cell morphologies and lacked a definitive transition zone, which suggests that secondary fiber differentiation was interrupted. In addition, the mutant exhibits severely reduced photoreceptors and a reduction in the number of horizontal cells at 7 dpf. Other retinal cell classes appeared unaffected in the mutant. Transmission electron microscopy and opsin immunohistochemistry showed that the different photoreceptor types were generated at the retinal margin, but the rods and cones failed to mature and disappeared. The mutant lens and retina also displayed increased cell proliferation based on proliferating cell nuclear antigen immunolabeling, suggesting that the lens opacity was due to unregulated cell proliferation and undifferentiated cell accumulation within the mutant lens. The lop mutant phenotype supports recent studies showing the lens has a role in regulating teleost retinal development. Developmental Dynamics 233:52–65, 2005. © 2005 Wiley‐Liss, Inc.