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Ectopic expression of Kit D814Y in spermatids of transgenic mice, interferes with sperm morphogenesis
Author(s) -
Schnabel Denhí,
Ramírez Laura,
Gertsenstein Marina,
Nagy Andras,
Lomelí Hilda
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20292
Subject(s) - spermiogenesis , biology , ectopic expression , microbiology and biotechnology , spermatid , transgene , germ cell , genetically modified mouse , spermatogenesis , genetics , endocrinology , gene , nucleus
Kit is a receptor tyrosine kinase that plays a fundamental role during the development of germ cells. Additionally, a truncated product, tr‐kit, expressed in haploid spermatids and mature spermatozoa can induce parthenogenetic activation when microinjected into mouse eggs, through the activation of PLCγ‐1. In this work, we induced ectopic expression of a mutated Kit protein, Kit D814Y during germ cell development. The in vivo expression of this mutant in spermatids produced malformations in mature spermatozoa, and in the most severe cases, sterility. Ultrastructural analysis indicated that condensing spermatids in the transgenic mouse presented a mislocalization of the manchette; a structure that has a crucial role during the elongation steps of spermiogenesis. This morphogenetic phenotype was accompanied by an increased phosphorylation of PLCγ‐1 in spermatogenic cells. Interestingly, we also found that, in wild‐type testis, PLCγ‐1 is specifically phosphorylated in condensing spermatids, coincident with the timing of expression of tr‐kit in spermiogenesis. We propose that alterations of PLCγ‐1 activity artificially promoted by ectopic Kit D814Y expression are related to the abnormalities of spermiogenesis. Our observations suggest that PLCγ‐1 activity could be involved in the shaping of spermatozoa. Developmental Dynamics 233:29–40, 2005. © 2005 Wiley‐Liss, Inc.