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Expression of a novel zebrafish zinc finger gene, gli2b , is affected in Hedgehog and Notch signaling related mutants during embryonic development
Author(s) -
Ke Zhiyuan,
Emelyanov Alexander,
Lim Simon Eng Seng,
Korzh Vladimir,
Gong Zhiyuan
Publication year - 2005
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20242
Subject(s) - gli2 , biology , zebrafish , hindbrain , hedgehog , notch signaling pathway , zinc finger , microbiology and biotechnology , gli3 , hedgehog signaling pathway , genetics , smoothened , gene , gastrulation , neural development , sonic hedgehog , transcription factor , embryonic stem cell , repressor
Abstract Gli zinc‐finger proteins are known as downstream mediators of the evolutionary conserved Hedgehog pathway. In zebrafish, gli2 functions differently from Gli2 in mammals. This difference could be due to the gli2 duplication in teleosts evolution and partial redundancy between two duplicated genes. Here, we report a novel zebrafish gli2 ‐like cDNA. Its structure, genetic location, and distinct expression pattern in the central nervous system suggested that this gene might represent a second gli2 of teleosts, and we named it gli2b . gli2b was expressed in the neural keel, excluding the forebrain–midbrain boundary, while gli2 expression complemented this pattern. After 24 hours postfertilization, several specific domains of gli2b expression were observed in the lateral and medial hindbrain and hypothalamus. In mutants affecting the Hedgehog and Notch signaling pathways, gli2b expression was either disrupted or extended in different regions. Developmental Dynamics 232:479–486, 2005. © 2005 Wiley‐Liss, Inc.