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Ventral otic cell lines as developmental models of auditory epithelial and neural precursors
Author(s) -
LawokoKerali G.,
Milo M.,
Davies D.,
Halsall A.,
Helyer R.,
Johnson C.M.,
Rivolta M.N.,
Tones M.A.,
Holley M.C.
Publication year - 2004
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20187
Subject(s) - biology , neurod , microbiology and biotechnology , embryonic stem cell , neural cell , neuroblast , transcription factor , gata3 , progenitor cell , cell , neurogenesis , genetics , gene , stem cell
Abstract Conditionally immortal cell lines were established from the ventral otocyst of the Immortomouse at embryonic day 10.5 and selected to represent precursors of auditory sensory neural and epithelial cells. Selection was based upon dissection, tissue‐specific markers, and expression of the transcription factor GATA3. Two cell lines expressed GATA3 but possessed intrinsically different genetic programs under differentiating conditions. US/VOT‐E36 represented epithelial progenitors with potential to differentiate into sensory and nonsensory epithelial cells. US/VOT‐N33 represented migrating neuroblasts. Under differentiating conditions in vitro the cell lines expressed very different gene expression profiles. Expression of several cell‐ and tissue‐specific markers, including the transcription factors Pax2, GATA3, and NeuroD, differed between the cell lines in a pattern consistent with that observed between their counterparts in vivo. We suggest that these and other conditionally immortal cell lines can be used to study transient events in development against different backgrounds of cell competence. Developmental Dynamics 231:801–814, 2004. © 2004 Wiley‐Liss, Inc.