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Screen for genes differentially expressed during regeneration of the zebrafish caudal fin
Author(s) -
Padhi Bhaja K.,
Joly Lucille,
Tellis Patricia,
Smith Amanda,
Nanjappa Purushothama,
Chevrette Mario,
Ekker Marc,
Akimenko MarieAndrée
Publication year - 2004
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20153
Subject(s) - biology , blastema , zebrafish , fish fin , regeneration (biology) , gene , suppression subtractive hybridization , differential display , in situ hybridization , genetics , microbiology and biotechnology , gene expression , anatomy , fish <actinopterygii> , cdna library , fishery
Abstract The zebrafish caudal fin constitutes an important model for studying the molecular basis of tissue regeneration. The cascade of genes induced after amputation or injury, leading to restoration of the lost fin structures, include those responsible for wound healing, blastema formation, tissue outgrowth, and patterning. We carried out a systematic study to identify genes that are up‐regulated during “initiation” (1 day) and “outgrowth and differentiation” (4 days) of fin regeneration by using two complementary methods, suppression subtraction hybridization (SSH) and differential display reverse transcriptase polymerase chain reaction (DDRT‐PCR). We obtained 298 distinct genes/sequences from SSH libraries and 24 distinct genes/sequences by DDRT‐PCR. We determined the expression of 54 of these genes using in situ hybridization. In parallel, gene expression analyses were done in zebrafish embryos and early larvae. The information gathered from the present study provides resources for further investigations into the molecular mechanisms of fin development and regeneration. Developmental Dynamics 231:527–541, 2004. © 2004 Wiley‐Liss, Inc.