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Telomerase activity and differential expression of telomerase genes and c‐myc in chicken cells in vitro
Author(s) -
Swanberg Susan E.,
Payne William S.,
Hunt Henry D.,
Dodgson Jerry B.,
Delany Mary E.
Publication year - 2004
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20149
Subject(s) - telomerase , telomerase reverse transcriptase , biology , telomere , embryonic stem cell , microbiology and biotechnology , gene , telomerase rna component , gene expression , cell culture , transcription factor , stem cell , reverse transcriptase , transcription (linguistics) , rna , genetics , linguistics , philosophy
This study examined telomerase activity and gene expression profiles for three genes in Gallus gallus domesticus : telomerase reverse transcriptase (chTERT), telomerase RNA (chTR), and c‐myc. Expression of these genes was studied in chicken embryonic stem (chES) cells, chicken embryo fibroblasts (CEFs), and DT40 cells using quantitative real‐time polymerase chain reaction. Our results establish that, relative to transcription levels in telomerase‐negative CEFs, chTERT and chTR are up‐regulated in telomerase‐positive chES cells. Transcription levels of chTERT, chTR, and c‐myc are dramatically up‐regulated in telomerase‐positive DT40 cells, relative to CEFs and chES cells. These results are consistent with a model in which telomerase activity is up‐regulated in proliferating embryonic stem cells requiring stable telomeres to endure multiple rounds of cell division; down‐regulated in differentiated, lifespan‐limited cells; and dramatically up‐regulated in immortalized, transformed cells for which uncontrolled proliferation is correlated with c‐myc dysregulation and telomerase activity. Developmental Dynamics 231:14–21, 2004. © 2004 Wiley‐Liss, Inc.