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PP1β9C interacts with trithorax in Drosophila wing development
Author(s) -
Rudenko Andrey,
Bennett Daimark,
Alphey Luke
Publication year - 2004
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20146
Subject(s) - biology , polytene chromosome , genetics , drosophila (subgenus) , chromatin , mutant , drosophila melanogaster , transcription factor , drosophilidae , microbiology and biotechnology , bromodomain , epigenetics , wing , gene , engineering , aerospace engineering
Type 1 Ser/Thr protein phosphatase (PP1) has many roles in Drosophila : regulating diverse processes from chromatin condensation to transforming growth factor‐β signaling. The presence of four PP1 genes, PP1 α 87B , PP1 β 9C , PP1 α 96A , and PP1 α 13C , encoding very similar proteins complicates analysis of their particular functions. Here, we report that the minor PP1 isoform PP1β9C binds in vitro and in vivo and genetically interacts with Trithorax (TRX), the archetypal member of the Trx‐G family of epigenetic regulators in Drosophila . Direct binding was demonstrated by GST pull‐down experiments and PP1β9C/TRX interaction in vivo was confirmed by coimmune precipitation from Drosophila embryonic extracts. PP1β9C was found to be present at all TRX sites on the polytene chromosomes. Flies homo‐ and hemizygous for loss‐of‐function alleles of PP1 β 9C exhibited specific wing defects when combined with various trx mutants, which indicates that PP1β9C and TRX cooperate in Drosophila wing development. Developmental Dynamics 231:336–341, 2004. © 2004 Wiley‐Liss, Inc.