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Bidirectional inhibitory interactions between the embryonic chicken metanephros and lumbosacral nerves in vitro
Author(s) -
Silver Lee,
Qiang Liang,
Loudon Robert,
Gallo Gianluca
Publication year - 2004
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20111
Subject(s) - biology , dorsal root ganglion , inhibitory postsynaptic potential , embryonic stem cell , sciatic nerve , microbiology and biotechnology , anatomy , axon , neuroscience , dorsum , biochemistry , gene
During chicken embryonic development the metanephros forms from the uretic duct at embryonic day (E) 7. As the metanephric tissue develops between E7 and E10, it comes into close apposition with lumbosacral nerves. Coculturing of metanephric and nerve explants demonstrated that the Schwann cells of the sciatic nerve inhibit the migration of metanephric cells in a contact‐dependent manner. Conversely, metanephric cells inhibit dorsal root ganglion axon extension in a contact‐dependent manner. However, metanephric cells are not inhibited by contact with growth cones or axons. Dorsal root ganglion growth cones become sensitive to the inhibitory signals on the surfaces of metanephric cells around E8, a time when the metanephros is expanding into the territory occupied by nerves in vivo. These observations demonstrate inhibitory bidirectional tissue–tissue interactions in vitro and provide a novel model system for the study of contact‐based guidance of both neuronal and non‐neuronal cell migration. Developmental Dynamics 231:190–198, 2004. © 2004 Wiley‐Liss, Inc.