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Establishment of embryonic stem cell lines from cynomolgus monkey blastocysts produced by IVF or ICSI
Author(s) -
Suemori Hirofumi,
Tada Takashi,
Torii Ryuzo,
Hosoi Yoshihiko,
Kobayashi Kinji,
Imahie Hiroshi,
Kondo Yasushi,
Iritani Akira,
Nakatsuji Norio
Publication year - 2001
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.1191
Subject(s) - biology , embryonic stem cell , embryoid body , ectoderm , stem cell , blastocyst , kosr , microbiology and biotechnology , endoderm , immunology , induced pluripotent stem cell , amniotic epithelial cells , cellular differentiation , andrology , adult stem cell , embryo , embryogenesis , genetics , medicine , gene
Human embryonic stem (ES) cells are predicted to be a valuable source for producing ES‐derived therapeutic spare tissues to treat diseases by controlling their growth and differentiation. To understand the regulative mechanisms of their differentiation in vivo and in vitro, ES cells derived from nonhuman primates could be a powerful tool. We established four ES cell lines from cynomolgus monkey ( Macaca fascicularis ) blastocysts produced by in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The ES cells were characterized by the expression of specific markers such as alkaline phosphatase and stage‐specific embryonic antigen‐4. They were successfully maintained in an undifferentiated state and with a normal karyotype even after more than 6 months of culture. Pluripotential competence was confirmed by the formation of teratomas containing ectoderm‐, mesoderm‐, and endoderm‐ derivatives after subcutaneous injection into SCID mice. Differentiation to a variety of tissues was identified by immunohistochemical analyses using tissue‐specific antibodies. Therefore, we established pluripotent ES cell lines derived from monkeys that are widely used as experimental animals. These lines could be a useful resource for preclinical stem cell research, including allogenic transplantation into monkey models of disease. © 2001 Wiley‐Liss, Inc.