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Isolation and expression analysis of three zebrafish angiopoietin genes
Author(s) -
Pham Van N.,
Roman Beth L.,
Weinstein Brant M.
Publication year - 2001
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.1157
Subject(s) - biology , zebrafish , mesenchyme , angiopoietin receptor , angiopoietin , mesoderm , microbiology and biotechnology , dorsal aorta , gastrulation , receptor tyrosine kinase , anatomy , myotome , embryonic stem cell , embryogenesis , receptor , embryo , gene , genetics , somite , signal transduction , vascular endothelial growth factor , cancer research , vegf receptors
The Tie1 and Tie2 receptor tyrosine kinases and the Tie2 ligands, the angiopoietins, play critical roles in vertebrate vascular embryogenesis, helping to mediate the interaction between endothelial cells and the pericytes or vascular smooth muscle cells that envelop and support them. We have obtained full‐length cDNA sequences for zebrafish orthologs of angiopoietin‐1 ( ang1 ), angiopoietin‐2 ( ang2 ), and angiopoietin‐like‐3 ( angptl3 ). Ang1 is expressed in head ventral mesenchyme, in the ventromedial region of somites, in mesenchyme surrounding trunk axial vessels, and in the hypochord, a transient embryonic structure of endodermal origin that has been implicated in dorsal aorta assembly in both zebrafish and Xenopus . Ang2 is expressed in head and anterior trunk ventral mesenchyme and the developing pronephric glomeruli. Angptl3 is expressed in the yolk syncytial layer. © 2001 Wiley‐Liss, Inc.