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Expression of zebrafish Lc3 synthase gene in embryonic lens requires hedgehog signaling
Author(s) -
Cao Ying,
Zhao Jue,
Wang Ying,
Meng Anming
Publication year - 2003
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.10372
Subject(s) - biology , zebrafish , microbiology and biotechnology , morpholino , hedgehog , polyketide synthase , hedgehog signaling pathway , in situ hybridization , mutant , embryo , gene expression , genetics , gene , signal transduction , biosynthesis , polyketide
Abstract Glycosyltransferases are involved in synthesis of various glycolipids and glycoproteins that play important roles in many biological processes. We have identified a zebrafish gene encoding a member of β1,3‐ N ‐acetylglucosaminyltransferase family, the Lc3 synthase/bGn‐T5. Whole‐mount in situ hybridization reveals that the Lc3 synthase gene is expressed in two distinct phases during the zebrafish embryogenesis. The early phase extends from late blastulation to the completion of epiboly, during which the expression occurs in the superficial layer of the embryos. The second phase of expression starts during mid‐segmentation and persists until day 3, during which the expression occurs prominently in the developing lens. The expression of the Lc3 synthase gene in the lens is inhibited in you‐too ( yot ) mutant embryos that are defective in Hedgehog signaling. The expression in the lens also decreases in cyclops ( cyc ) and one‐eyed‐pinhead ( oep ) mutant embryos and lefty1 ‐injected embryos, which are deficient in Nodal signaling and lack Hedgehog activity in the ventral brain. These results suggest that Hedgehog signaling is required for the Lc3 synthase expression in embryonic lens. Developmental Dynamics, 2003. © 2003 Wiley‐Liss, Inc.